Introduction To Analysis Solution Manual

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Solution

In considering Figure 1-2, if you were to extend t he di agram, what would the next two stages of “magnification” beyond DNA be? Answer: The next stage of the diagram would be an in-depth look into the DNA molecule, as two long molecular strands of nucleotides wound around each other in a double helix and the basic structure of those monomers. Focus on specific nucleotides and their organic molecule parts: a deoxyribose sugar, a phosphate group, and a nitrogenous base (adenine, thymine, guanine, and cytosine). Second, would be atomic composition of those organic molecules (C, H, O, P, and N). In Figure 1-21, why are colored ladders of bands shown in all three electrophoretic gels?

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If the molecular labels used in all cases were radioactive, do you think the black bands in the bottom part of the figure would all be radioactive? Answer: In this figure we see three different types of electrophoresis in different colors (Southern blot of DNA fragments, Northern blot of RNA and Western blot of a protein product). Such a mixture of macromolecules could be hybridized with a radioactive probe, and the bands in the lower part of the figure would indicate radioactivity.